Thursday, September 5th 2013

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Class Date: Thursday, September 5th 2013

Dear blog and blog readers,

On September 5th in microbiology we obtained our streak plate from the incubator in hope that it had resulted in a pure culture.

In this streak plate we examined mostly circular bacteria (form) with some filamentous (which is seen more clearly in the first microscope image). The bacteria was raised, and there was mostly red, opaque bacteria , with some cream, translucent bacteria and some yellow, opaque bacteria. 

Max proudly holding our first streak plate. 

We looked at our steak plate of the soil sample under the microscope and here are some images we viewed.

In this image the filamentous form of the bacteria is clearly observed. 

Here reveals the colors we observed rather well (cream and red). 

The form (circular) as well as the color of the bacteria (red and cream) are observed here. 

Our streak plate did in fact result in a pure culture, but Dr. P thought it would be interesting to see how the pure culture grew at 3 different temperatures.


Therefore we made 3 more streak plates from simply the pure culture of the red pigmented bacteria. We placed them in the incubators that were at the temperatures 25 degrees Celsius (room temperature), 30 degrees Celsius (air temperature), and 37 degrees Celsius.

We hypothesized that the colder the incubator was the less the bacteria would grow due to our belief that cold can inhibit bacteria growth.

Therefore, we assumed that the streak plate in the 25 degree incubator would be contain less bacteria, the streak plate in the 30 degree incubator would contain the same amount of bacteria as our original streak plate (our original streak plate was incubated at 30 degrees Celsius), and that the streak plate in the 37 degree incubator would contain the most abundant bacteria.

That's all for today! Check back soon to see how are bacteria is growing....