Tuesday, September 3rd 2013

*This blog does not reflect Franciscan University in any way*

Class date: Tuesday, September 3rd 2013

Dear Blog and Blog readers,

Today we analyzed our petri dishs wich contained our bacteria samples from the Ohio river. This included our soil sample, and bucket sample. Both of which were highly contaminated, however, the soil sample contained some bacteria that was producing a red pigment.

                                          Soil Sample:

                                          Bucket Sample:

We also had to read the lab procedure to determine how to create a streak plate. The purpose of this was to isolate bacterial colonies in order to create a pure culture of one colony of bacteria.

First we were given a new agar plate that would become the streak plate.

We prepared and lit the bunsen plate and sterilized the loop. We touched the loop to each color of bacteria in the plate contaminated with the bacteria from the soil and then we streaked the bacteria by moving the tip of the loop contaminated in a back and forth motion on one quadrant of the new agar plate. We then sterilized the loop and put the edge of the loop on the already streaked quadrant and streaked a new quadrant. We repeated this until all four quadrants were streaked, sterlizing the loop between each rotation. When we streaked the final quadrant we did a wave motion leading to the middle of the plate.

 

We then stored this in the incubator at the temperature the sample was taken (30 degrees Celsius). 

We then looked at our soil sample under a light microscope and this was the image we saw. 

We put the soil sample and the bucket sample into the refrigerador so that the bacteria would not continue to grow. 

We predicted that the streak plate would result in the red, yellow, and beige color bacteria being separated and from there we would be able to take a pure culture. 

Tune in next week to find out what we....find out.